Cancer Cell Biology: human and Flow Cytometry: BD Flow Calibur,
mouse cell culture, gene Canto II, Accuri C6, surface
transfection, cell cycle, cell marker staining, intra cellular
proliferation assay, apoptosis, cell staining, multiple color staining,
signaling pathway, etc. FlowJo, FCS Express.
Transgenic Mice Model: colony Immunology: T cell, B cell,
breeding , genotyping, adoptive immune cell transfer, TIL
Xenograft, micro-dissection, etc. isolation, cytokine assay, etc.
Molecular and biochemical Cancer Stem Cell: Isolation, stem
biology: DNA, RNA, protein, real cell culture, sphere
time PCR, gene microarray, assay, tumorigenicity, tissue
cytokine array, Western Blot, regeneration, etc.
Northern Blot, Southern Blot, etc. Bio-statistics analysis: GraphPad ,
IHC and H & E: tissue staining, ANOVA , etc.