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Skillful Research Associate resume

JIANSHENG WU
Accomplishments
  • Project 1- Diet(omega-3 & omega-6) and Prostate Cancer- PTEN and LOX or COX double knockout mice model Project 2 - Inflammation and Prostate Cancer (1)Rag1 and PTEN double knockout mice model, Bone marrow adoptive transfer (2)CD4 and PTEN double knockout mice model (3)CD8 and PTEN double knockout mice model (4)Jh and PTEN double out mice model Project 3 - Dip13beta(APPL2) gene konockout mice Project 4 - PTEN knockout and p53 mutation and Prostate Cancer Metastasis Project 5 High Myc mice model -Prostate cancer Project 6 PERK and Prostate cancer-PTEN and PERK double knockout Project 7 SDC1 and Prostate cancer-PTEN and SDC1 double knockout Project 8 Prostate cancer stem cell- stem cell isolation from FASN and.
Experience
02/2011 to Current
Lab Manager Wake Forest University School of Medicine Winston Salem, NC
  • 1) Lab management: MS access inventory database system for chemical, cell line, antibody), PeopleSoft ordering, equipment maintenance ,etc.
  • 2)Training and supervision of lab personnel (Ph.D student, lab technician).
08/2004 to Current
Research Associate Wake Forest University School of Medicine Winston Salem, USA, NC
04/2002 to 07/2004
Post-Doctoral Research Fellow Wake Forest University School of Medicine Winston Salem, USA, NC
  • ABCA1 and PTEN Triple knockout mice, sphere assay, tumorigenecity, tissue regeneration.
  • Dip13alpha(APPL1) and Dip13beta (APPL2) and cell cycle arrest.
03/2001 to 03/2002
Research Scholar North Carolina State University Raleigh, USA, NC
  • Soil microbe and plant pathology.
Research Associate Lab Manager Wake Forest University School of Medicine Winston Salem, USA, NC
  • I have successfully shifted my studies from the agricultural field to the medical field after I got my PhD in 2000.
  • Because I am very interested in cancer research, I have continued working in that area for more than 15 years.
  • My research focus is on prostate cancer.
  • Through these many years of research experience, I have developed an excellent proficiency in cancer research with broad scopes of both in vitro and in vivo, from cell cultures to animal models.
  • My strongest skills include adapting, developing, and troubleshooting.
  • For a new or tough project, I am able to figure out the key issue by searching the.
Education and Training
2000
Ph.D: Molecular Plant Pathology Nanjing Agricultural University Nanjing, Jiangsu Province, China Molecular Plant Pathology
1993
Master of Science: Molecular Plant Pathology Nanjing Agricultural University Nanjing, Jiangsu Province, China Molecular Plant Pathology
1988
Bachelor of Science: Biology Anhui Normal University Wuhu, Anhui Province, China Biology
Interests
Wang, J.S.,Wen, W.G,, Lu, X.Z., Song, C.F. Wu, J.S. A protein elicitor for biocontrol of plant diseases and pests.(file number: CN1251371A) Wang, J.S., Wu, J.S., Dong, H.S Bacillus subtilis strain as biocontrol agent of plant disease.(file number:CN1237346A) Wang, J.S, Liu, J.J, Wu, J.S, Fang, H., Wei, Z.M. The small molecular bacteriocin from Erwinia chrysanthemi and its fermentation technique and separation method.(file number:CN1245164
Activities and Honors
Cancer Cell Biology: human and Flow Cytometry: BD Flow Calibur, mouse cell culture, gene Canto II, Accuri C6, surface transfection, cell cycle, cell marker staining, intra cellular proliferation assay, apoptosis, cell staining, multiple color staining, signaling pathway, etc. FlowJo, FCS Express. Transgenic Mice Model: colony Immunology: T cell, B cell, breeding , genotyping, adoptive immune cell transfer, TIL Xenograft, micro-dissection, etc. isolation, cytokine assay, etc. Molecular and biochemical Cancer Stem Cell: Isolation, stem biology: DNA, RNA, protein, real cell culture, sphere time PCR, gene microarray, assay, tumorigenicity, tissue cytokine array, Western Blot, regeneration, etc. Northern Blot, Southern Blot, etc. Bio-statistics analysis: GraphPad , IHC and H & E: tissue staining, ANOVA , etc. evaluation, etc.
Publications
Suburu, J., Shi, L.H., Wu, J.S, Wang, S.H., Samuel, M., Thomas, M.J., Kock, N.D., Yang, G.Y., Kridel, S., and Chen, Y.Q. Fatty acid synthase is required for mammary gland development and milk production during lactation. Am J Physiol Endocrinal Metab 2014 306:E1132-E1143. *Gu, Z.N., Wu, J.S, Wang,S.H., Suburu, J., Chen, H.Q., Thomas, M.J., Shi, L.H., Edwards,I.J, Berquin, I.M., Chen, Y.Q. Polyunsaturated fatty acids affect the localization and signaling of PIP3/AKT in prostate cancer cells 2013 Carcinogenesis 2013 34(9):1968-1975 *Wang, S.H., Wu, J.S, Suburu, J., Gu, Z.N., Cai, J., Axanova, L.S., Cramer, S.D., Thomas, M.J., Perry, D.L., Edwards, I.J., Mucci, L.A., Loda, M.F., Sui, G.C., Berquin, I.M., Chen, Y.Q. Effect of dietary polyunsaturated fatty acids on castration- resistant prostate cancer Carcinogenesis. 2012 Feb;33(2):404-12 *Wang, L, Chen, W, Feng, Y, Ren Y, Gu, Z, Chen, H, Wang, H, Thomas, M.J., Zhang, B., Berquin, I.M., Li, Y., Wu, J.S. , Zhang, H., Song, Y., Liu, X., Norris, J.S., Wang, S., Du, P., Shen, J., Wang, N., Yang, Y., Wang, W., Feng, L., Ratledge, C., Zhang, H., Chen, Y.Q. Genome characterization of the oleaginous fungus Mortierella alpina. PLoS One. 2011;6(12):e28319. *Hu, Y.P, Sun, H.G, Owens, R.T, Gu Z.N., Wu, J.S., Chen, Y.Q., O'Flaherty J.T., Edwards, I.J.. Syndecan-1-dependent suppression of PDK1/Akt/Bad signaling by docosahexaenoic acid induces apoptosis in prostate cancer. Neoplasia. 2010 Oct;12(10):826-36. *Hu, Y.P, Sun, H.G, Owens, R.T, Wu, J.S., Chen , Y.Q., Berquin, I.M., Perry, D.L O'Flaherty J.T., Edwards, I.J. Decorin suppresses prostate tumor growth through inhibition of epidermal growth factor and androgen receptor pathways. Neoplasia. 2009 Oct;11(10):1042-53. *Berquin, I.M., Min, Y.N., Wu, R.P., Wu, J.S., Perry, D., Cline, J.M., Thomas, M.J., Thornburg, T., Kulik, G., Smith, A., Edwards, I.J., D'Agostino, R., Zhang ,H., Wu ,H., Kang, J.X., Chen, Y.Q. Modulation of prostate cancer genetic risk by omega-3 and omega-6 fatty acids. Journal of Clinical Investigation 2007 Jul;117(7):1866-75. *Hu, S.J., Wu, J.S., Burkey, K.O., Firestone, M.K. Plant and microbial N acquisition under elevated atmospheric CO2 in two mesocosm experiments with annual grasses. Global Change Biology 2005 11( 2) : 213-223 *Zhang, W.J., Feng, J.X., Wu, J.S., Parker, K. Differences in soil microbial biomass and activity for six agroecosystems with a management disturbance gradient. Pedosphere 2004 14(4): 441-447. *Wu, J.S., Lu, X.Z., Shao, M., Wang, J.S. A bacterium possessing biological activity against toxins in organic acids produced by phytopathogen. Journal of Southwest Agricultural University.2000 22 (3): 222-225. (This paper was abstracted in the CAB international 2000/08-2001/10) *Wu, J.S., Wang J.S. Control effect of W-1 with detoxification activity activity against Scleotinia sclerotiorum and its primary identification Journal of Southwest Agricultural University 2000 22(6):487-489. *Wang J.S., Wu, J.S, Song C.F and Liu F.Q. 2000. Phytopathogenic Bacteriology. China Agriculture Press(Book) *Zhu, Y.L., Wu J.S., Wang, J.S. Analysis of resistance-related proteins in rice against Xanthomonas oryzae pv.oryzae by two-dimensional electrophoresis Scientia Agricultura Sinica 2000 33(4): 91-93. (This paper was abstracted in the CAB international 1996-1998/07) *Wu, J.S., Liang, J.D., Wang, J.S. Effects of Echcin on control of crop fungal and bacterial diseases. Acta Phytopathologica Sinica. 1999 29:104-109. (This paper was abstracted in the CAB international 1998/08-2000/07) *Li, H.Y., Wu, J.S, Song, C.F. and Wang, J.S. Hypersensitive reaction in the interaction between rice IR26 cell suspension and Xanthomonas oryzae pv. oryzae Acta Phytopathologica Sinica. 1999 29(4):299-303.(This paper was abstracted in the CAB international 1998/08-2000/07) *Ouyang, P.K., Zhang, G..D., Wu, J.S., Wang, J.S., Zhang, L.Y., Li, G.F. 1999. Handbook of Chemical Industrial Products. Chemical Industry Press(Book). *Wu, J.S. , Wang, J.S. Changes in soluble proteins in rice leaves in the interaction between rice and bacterial blight (Xanthomonas oryzae pv. oryzae). Journal of Southwest Agricultural University 1998 20(4): 321-327. (This paper was abstracted in the CAB international 1998/08-2000/07) *Yang, Z. C., Wu, J.S.1998. Screening of biocontrol microorganism against two wheat diseases. Chinese Journal of Microecology(supplement)10: 61-64. *Zhu, Y.L., Wu, J.S. Wang, J.S. A preliminary study on the expression of defense genes in rice-suspension induced by Xanthomonas oryzae pv.oryzae. Acta Phytopathologica Sinica 1997 29: 250. (This paper was abstracted in the CAB international 1996-1998/07) *Wu, J.S., Dong, C., Leng, F.F. Wang, J.S. Induced resistance of the incompatible race of Xanthomonas oryzae pv.oryzae to rice and preliminary study of PR-proteins. Acta Phytopathologica Sinica 1996 26(2):110. (This paper was abstracted in the CAB international 1996-1998/07) *Wu, J.S., Wang, J.S. The bacteriocin from the phytopathogenic bacteria 1996 Microbiology 23:95-101. *Liu, H.L., Zhang, X.Z, Wang, J.S. Wu, J.S..Purification and characterization of antifungal protein of B3(Bacillus subtilis) Journal of Agricultural biotechnology 1995 26:33-38.
Personal Information
references and analyzing critically. This way, I can tackle the problems and work out the best possible solutions. That is why I may forge ahead in the field of cancer research even when it was not my original expertise. My specially developed research results have provided the strong evidence necessary to help my PI successfully receive NIHR01 funding in 2012.
Additional Information
  • references and analyzing critically. This way, I can tackle the problems and work out the best possible solutions. That is why I may forge ahead in the field of cancer research even when it was not my original expertise. My specially developed research results have provided the strong evidence necessary to help my PI successfully receive NIHR01 funding in 2012.
  • PATENTS Wang, J.S.,Wen, W.G,, Lu, X.Z., Song, C.F. Wu, J.S. A protein elicitor for biocontrol of plant diseases and pests.(file number: CN1251371A) Wang, J.S., Wu, J.S., Dong, H.S Bacillus subtilis strain as biocontrol agent of plant disease.(file number:CN1237346A) Wang, J.S, Liu, J.J, Wu, J.S, Fang, H., Wei, Z.M. The small molecular bacteriocin from Erwinia chrysanthemi and its fermentation technique and separation method.(file number:CN1245164A) HONORS *Caner Biology Honor, Department of Cancer Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA (2011). *Science and Technology Advance Award, Jiangsu Province Science and Technology Committee, Jiangsu Province, P.R.China (1999). *Excellent Research Paper Award, Nanjing Agricultural University, Jiangsu Province, P.R.China (1997).
Skills
alpha, Biology, cancer, database, equipment maintenance, focus, inventory, MS access, 2000, pathology, PeopleSoft, personnel, PhD, research, supervision, technician, troubleshooting
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Job-winning Research Associate resume

Yasamin Khorramzadeh - Physics Phd.
Professional Summary
Skilled Research scientist with a strong math background and experience in modeling, machine learning, and statistics. Specialized in collecting and analyzing data to detect trends and build models to solve a variety of complex problems to extract actionable insights that will help decision making and policy development.
Skills
  • Statistics & Predictive Modeling
  • Machine Learning
  • Network Analysis
  • Natural Language Processing
  • Critical & Analytical Skills
  • Experience in Distributed Computing Spark
  • Code Writing Capabilities
  • Exceptional Research & Organizational Skills
  • Detail Oriented Problem Solver
  • Excellent Communication Skills
Software and Programming Languages
  • Python (scikit-learn, numpy, scipy, pandas, genism, Networkx), SQL, Tensorflow, Spark, Google Cloud, Github, Linux, LaTeX
Work History
Postdoctoral Research Associate, 03/2016 to Current
Northeastern University
  • Conducted end-to-end analysis, from data curation and disambiguation, to feature engineering and model building to explain and predict success in academic community.
  • Extracted data from MySQL large-scale (Terabytes) datasets and non-structured data through web scraping, wrote scripts to parse, clean, combine, and process them.
  • Used linear models for regression analysis, Hierarchical clustering for population segmentation, and decision tree ensembles to gain insight on the most important factors.
  • Drew inferences and conclusions, communicated the results through reports, charts, or tables.
Topic modeling/Network analysis/Recommender Systems, 06/2017 to Current
Northeastern University
  • Topic modeling:   Used LDA to find the topics presented at NIPS'15 (CS conference). Scrapped the web for citation counts of the papers. Found the most impactful topics from NIPS'15 and used T-SNE to visualize.
  • Network analysis: Constructed collaboration network at NIPS'15. Used K-mean clustering to classify authors based on their career stage and visualized the network using Networkx and Plotly modules.
  • Recommender Systems: Used the rating information from goodreads website to implement the state-of-the-art matrix factorization and Deep Neural Networks and built recommender systems using both Spark and Tensoreflow.
Education
Ph.D.: Physics, 2015
Virginia Polytechnic Institute and State University - Blacksburg, VA
Bachelor of Science: Physics, 2009
Sharif University of Technology - Tehran, Iran
Selected Publications and Side Projects
  • Boson Core Compressibility” Phys. Rev. A (2012)
  • Analyzing network reliability using structural motifs, Phys. Rev E. (2015)
  • Network Reliability: The effect of local network structure on diffusive processes Phys. Rev E (2013)
  • Using the network reliability polynomial to characterize and design networks, Journal of Complex Networks 2 (4), 356-372, (2014)
  • Network literacy project: collaborated in initiating a program aiming to introduce the basic concepts of network science targeting secondary education.
  • Lead scientist in the Defense Advanced Research Projects Agency (DARPA)  grant “Accelerating Science by Separating Performance Measures from Network Effects”, with Albert-László Barabási Sep 2016.
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Experienced Research Associate resume

Bhaskar Kahali
Work History
Postdoctoral Research Associate, 12/2014 to Current
St Jude Children's Research Hospital Memphis, TN
  • Transplant & Cellular Therapy (PI - Lea Cunningham, MD).
    • Conducted basic and applied research on Epigenetic regulation of Tumor Microenvironment.
    • Direct Laboratory personnel, train, and monitor performance as the senior member of the laboratory.
    • Collected and analyzed biological data.
    • Designed and optimized assays.
    • Store, process and analyze data.
    • Write manuscripts for scientific publications.
    • Interpreted research findings and summarized data into reports.
    • Attended scientific conferences to present ongoing research in the laboratory.
    • Facilitated research into developing effective therapy of disease.
    • Ordered laboratory equipment and supplies.
Postdoctoral Fellow, 12/2010 to 12/2014
Postdoctoral Fellowship, University of Florida, College of Medicine University of Florida Gainesville, FL.
  • Honors 2013 Awarded the highest impact publication (Oncogene, Kahali et.al.
  • Conducted basic and applied research on Cancer Epigenetics.
  • Direct Laboratory personnel, train, and monitor performance as the senior member of the laboratory.
  • Collected and analyzed biological data.
  • Designed and optimized assays.
  • Store, process and analyze data.
  • Write manuscripts for scientific publications.
  • Interpreted research findings and summarized data into reports.
  • Attended scientific conferences to present research.
  • Worked with several teams on Cancer Epigenetic research.
  • Facilitated research into developing effective therapy of disease.
  • Ordered laboratory equipment and supplies.
Education
Ph.D.: Molecular Biology, 2010
West Virginia University - Morgantown, WV
Molecular Biology
Master of Science: Zoology (Specialization in Genetics & Molecular Biology), 2002
University of Kolkata - Kolkate, India
Zoology (specialization: Genetics & Molecular Biology) A. Positions and Honors.
Publications

1. Evaluation of CD123-specific chimeric antigen receptor (CAR)-engineered natural killer cells for treating high-risk acute myeloid leukemia

C. Sinha, A. Seth, B. Kahali, K-Nam Koh, L. Cunningham

Cytotherapy (Under revision).

2. Flavonoids from each of the six structural groups reactivate BRM, a possible cofactor for the anticancer effects of Flavonoids.

B.Kahali*, S.B. Marquez*, K.W. Thompson, J. Yu, S.J. Gramling, L. Liu, A. Aponick, D. Reisman

(* - Equal Contribution)

[Carcinogenesis. 2014 Oct:35(10):2183-93]

 

3. The silencing of the SWI/SNF subunit and anticancer gene BRM in Rhabdoid tumors.

B. Kahali, J. Yu, S.B. Marquez, K.W. Thompson, S.Y. Liang, L. Lu, D. Reisman [Oncotarget. 2014 May 30;5(10):3316-32].


4. Identifying targets for the restoration and reactivation of BRM.

B.Kahali, S.J.B. Gramling, S.B. Marquez, K. Thompson, L. Lu, D. Reisman [Oncogene 2014 Jan 30:33(5):653-64]

[Awarded the highest impact publication in 2012, Department of Medicine, University of Florida]


5. Downregulation of SWI/SNF chromatin remodeling factor subunits modulates cisplatin cytotoxicity.

A. Kothandapani, K. Gopalakrishnan, B. Kahali, D. Reisman, S. M. Patrick

[Exp Cell Res. 2012 Oct; 318(16): 1973-86.]


6. Evidence that the C-terminal domain (CtD) autoinhibits neural repression by Drosophila E(spl)M8.

B.Kahali, J.E. Kim, U. Karandikar, C.P. Bishop, A.P. Bidwai.

[Genesis-2010 Jan;48(1):44-55]


7. Functional Dissection of Timekeeper (Tik) implicates opposite roles for CK2 and PP2A in Drosophila neurogenesis.

E. Kunttas-Tatli, A. Bose, B. Kahali, C.P. Bishop, A.P. Bidwai [Genesis. 2009 Oct;47(10):647-58].


8. On the mechanism underlying the divergent retinal and bristle defects of M8*(E(spl)D) in Drosphila B. Kahali, A. Bose, U. Karandiakar, C.P. Bishop, A.P. Bidwai [Genesis. 2009 Jul;47(7):456-68.]


9. Drosophila CK2 phosphorylates Hairy and regulates its activity in vivo. B. Kahali, R. Trott, Z. Paroush, R. Allada, C.P. Bishop, A.P. Bidwai [Biochem Biophys Res Commun. 2008 Sep 5;373(4):637-42.]


10. Drosophila CK2 regulates lateral-inhibition during eye and bristle development A. Bose, B. Kahali, S. Zhang, JM Lin, R. Allada, , U. Karandikar, A.P. Bidwai [Mech Dev. 2006 Sep;123(9):649-64.]


 

Published Abstracts and Presentations.


1. Abstract/Poster Discussion Session: ASH Annual Meeting and Exposition, 2016. SWI/SNF Chromatin Remodeling Complex Regulates the Expression of PD-L1 in a Spatio-Temporal Manner in Acute Lymphoblastic Leukemia (ALL)

B. Kahali, C. Sinha, A. Ritter, L.C. Cunningham Blood 128 (22), 2918-2918


2. Abstract/Poster Discussion Session: ASH Annual Meeting and Exposition, 2016. An Evaluation of the Cytotoxic Effect of the Natural Product Aaptamine against t(4;11) Leukemias

C. Sinha, J. Bowling, A. Seth, B. Ju, B. Kahali, M Hamann, L.C. Cunningham Blood 128 (22), 1624-1624


3. Abstract/Poster Discussion Session: BMT Tandem Meetings, ASBMT, 2016. Modulation of NKG2D Ligands Expression in Acute Myeloid Leukemia

C. Sinha, B. Ju, S.S. Ong, A. Goktug, W. Lei,A. Seth, B. Kahali, T. Chen, W.H. Leung, L.C. Cunningham Biology of Bone Marrow Transplant 22 (3), S211


4. Abstract/Poster Discussion Session: BMT Tandem Meetings, ASBMT, 2016. CD62L and TCR Depletion of Donors in a Double Haploidentical BMT Model Leads to Improved Donor Engraftment

A. Seth, C. Sinha, B. Kahali, W. H. Leung, L.C. Cunningham Biology of Bone Marrow Transplant 22 (3), S355-S356


5. Abstract/Poster Discussion Session: AACR Chromatin and Epigenetics Conference, 2013. Epigenetic Silencing of Brahma, a Subunit of the Chromatin Remodeling Complex SWI/SNF

B. Kahali, S. Gramling, K. Thompson, S. Marquez, L. Lu, D. Reisman Cancer Research 73 (13 Supplement), A42-A42.


6. Abstract/Poster Discussion Session: AACR Annual Conference, 2012. Epigenetic Regulation of BRM

B.Kahali, S. Gramling, D.N. Reisman Cancer Research 72 (8 Supplement), 4609-4609


7. Poster Presentation: Genetic Society of America (GSA), Annual Drosophila Conference, 2010. Genetic interactions between CK2, E(spl), Notch and PP2A during Drosophila neurogenesis.

A.Bose, B. Kahali, C. Bishop, A. Bidwai


8. Poster Presentation: Genetic Society of America (GSA), Annual Drosophila Conference, 2010. Genetic Interactions of p38a, CK2 and Notch during Drosophila Eye Development

C. Yin, B. Kahali, A. Bose, S. Zhang, C. Bishop, A. Bidwai


9. Poster Presentation: Genetic Society of America (GSA), Annual Drosophila Conference, 2008. Analysis of a CK2 phosphorylation-specific variant of E(spl)M8 leads to a reinterpretation of the mechanism of E(spl)D.

B.Kahali, A. Bose, C. Bishop, A. Bidwai


10. Poster Presentation: Genetic Society of America (GSA), Annual Drosophila Conference, 2008. Analysis of the interactions between CK2, PP2A and E(spl) during neurogenesis.

A. Bose, E. Kunttas-Tatli, B. Kahali, C. Bishop, A. Bidwai


11. Poster Presentation: Genetic Society of America (GSA), Annual Drosophila Conference, 2006. Context-specific roles for functional domains of E(spl)M8 during bristle and eye development

B.Kahali, A. Bose, C. Bishop, A. Bidwai.

Technical Skills
  • Proficient in PCR, Cloning, Site-direted mutagenesis, homologous recombination based gene manipulations (TALEN and CRISPR/cas9)
  • Cell culture
  • Virus production and transduction
  • Protein purification
  • Western blot
  • Flow Cytometry
  • RNA isolation
  • RNA interference based gene silencing
  • Gene expression
  • ELISA assay
  • Chromatin Immunoprecipitation Assay (ChIP).
  • Immunohistochemistry assay
Additional Information
  • 2010 WVU Dissertation Fellowship 2008 WVU Travel Award (Awarded for presentation at the 49th Annual Drosophila Conference, Houston, TX) 2006 WVU Travel Award (Awarded for presentation at the 47th Annual Drosophila Conference, San Diego, CA) 2000-2002 National Scholarship Award, Govt. of India
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